Fluorometer… what?

Business / Business Services

Gepubliceerd door: Firma fair focus

So, you are interested in fluorometers, but it is not really clear to you what it is, and what can be done with it. That is not really weird, not many people are familiar with the advanced technologies that this product brings. Also, there are many factors and applications that need to be taken into account when using it. When deciding to buy a fluorometer, be sure to take a look at the Life Science Product of the Year.

What does a fluorometer do?

It is really logical to not be familiar with all the functionalities of a fluorometer. That is mainly the reason that it is possible that you are reading this article. A fluorometer provides the possibility to analyse whether a product or object has come in contact with an object that is not its own. The way that fluorescence is measured is:

Scanning the object, then making a sample of it, and filtering it. You are left with the light emission that matters. This goes through a detector and the last step that the fluorometer takes is showing a basic plan that is made up of individual molecules that are found.

Applications

Fluorescence behaviour is not something that is definite. It can be influenced by different applications.

The first factor that often influences this are ligand binding and conformational changes.

Another application, that in this case only works with chelating dyes, is calcium. The fluorescence is then increased.

Fluorescence Resonance Energy Transfer (or FRET) is a term that sounds more difficult than it is. It means that two objects come close to each other and one of them donates its light to the other, that causes the receiving one to become excited and starts to emit its own light.

Application is therefore one of the most important factors to be aware of, otherwise the results of research can easily be lost. A fluorometer is often very hard to use, so be sure to use it in the way it is supposed to.

Life Science Product of the Year

Reviews by professionals in life science decide what product wins the Life Science Product of the Year award and in this case it was a fluorometer. To be more specific, the winner was the spectrophotometer fluorometer DeNovix DS-11 FX+. Unlike other years, it was not even close, and thanks to its simplicity, the fluorometer won with ease.

Veelgestelde vragen

Wat is een fluorometer en wat doet het?▼

Een fluorometer is een apparaat dat analyseert of een product of object in contact is geweest met iets anders. Het scant het object, maakt een sample, filtert deze en toont een plan gemaakt van individuele moleculen die zijn gevonden.

Hoe werkt een fluorometer precies?▼

Een fluorometer scant het object, maakt er een sample van, filtert deze en laat het licht door een detector gaan. Vervolgens toont het apparaat de resultaten als een plan met individuele moleculen.

Wat zijn de toepassingen van fluorescence?▼

Fluorescence gedrag wordt beïnvloed door verschillende factoren zoals ligand binding, conformationele veranderingen en calcium. FRET is een techniek waarbij twee objecten licht uitwisselen en elkaar exciteren.

Welke fluorometer won de Life Science Product of the Year award?▼

De DeNovix DS-11 FX+ spectrophotometer fluorometer won de award vanwege zijn eenvoud en gebruiksgemak. Het verschil met andere kandidaten was zeer groot.

Is een fluorometer moeilijk te gebruiken?▼

Ja, een fluorometer is vaak lastig in gebruik. Het is belangrijk om het apparaat op de juiste manier te gebruiken, anders kunnen onderzoeksresultaten gemakkelijk verloren gaan.